Abstract
Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biosensing Techniques
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Ligands
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Mice
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Proteins / chemistry*
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Tosyl Compounds / chemistry*
Substances
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Ligands
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Proteins
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Tosyl Compounds
Associated data
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PubChem-Substance/57288313
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PubChem-Substance/57288314
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PubChem-Substance/57288315
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PubChem-Substance/57288316
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PubChem-Substance/57288317
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PubChem-Substance/57288318
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PubChem-Substance/57288319
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PubChem-Substance/57288320
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PubChem-Substance/57288321
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PubChem-Substance/57288322
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PubChem-Substance/57288323
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PubChem-Substance/57288324
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PubChem-Substance/57288325
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PubChem-Substance/57288326
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PubChem-Substance/57288327
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PubChem-Substance/57288328
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PubChem-Substance/57288329
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PubChem-Substance/57288330
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PubChem-Substance/57288331
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PubChem-Substance/57288332
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PubChem-Substance/57288333
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PubChem-Substance/57288334
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PubChem-Substance/57288335
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PubChem-Substance/57288336
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PubChem-Substance/57288337
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PubChem-Substance/57288338
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PubChem-Substance/57288339
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PubChem-Substance/57288340
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PubChem-Substance/57288341
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PubChem-Substance/57288342
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PubChem-Substance/57288343
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PubChem-Substance/57288344
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PubChem-Substance/57288345
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PubChem-Substance/57288346
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PubChem-Substance/57288347
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PubChem-Substance/57288348
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PubChem-Substance/57288349